Too Much L Glutamine Cell Culture

L glutamine serves as an auxiliary energy source especially when cells are rapidly dividing and also can be used by cells as a source of nitrogen for the synthesis of proteins nucleic acids etc.

Too much l glutamine cell culture.

L glutamine is an unstable essential amino acid required in cell culture media formulations. It is used extensively in wide range of media including classical media serum free media insect cell culture. It is produced by the body and is also found in food. Glutamine is an amino acid a building block for proteins found naturally in the body.

L glutamine is unstable at physiological ph in liquid media. L glutamine is supplied in both liquid and powdered forms to be used as an amino acid supplement for cell culture media. Powdered glutamine is supplied as a usp grade chemical and liquid formulations of glutamine are supplied at 200 mm 100x at a final concentration of 29 2 mg ml in 0 85 nacl. Glutamine is taken by mouth for sickle cell disease to improve nutrition and help people recover from.

Glutamine supplements may interact with chemotherapy medications making them more effective and helping to reduce their side effects according to the university of maryland medical center. The center however also notes that research is conflicting in this area and glutamine might also cause more tumors to grow. One study used advanced lab techniques to determine how much l glutamine is found in various foods. L glutamine is a non essential amino acid that is often simply called glutamine.

The following are the percentages of protein made up of l glutamine in each food. Most commercially available media are formulated with free l glutamine which is either included in the basal formula or added to liquid formulations at time of use. In cell culture systems l glutamine serves as a crucial component of culture media. However of greater physiological importance to many cells l glutamine is a precursor of l glutamate.